The flow cytometers Diaries

The flow cytometers Diaries

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Info-enriched characterization of cell populations. Morphological details from visuals can insert to your richness of flow cytometry facts. By way of example, the determine displays an in any other case conventional apoptosis assay making use of Annexin V and PI, adding cell imaging to characterize cells in each population to reveal morphologically distinct features. These insights could not are already received from multiplex staining by yourself.

As samples are obtained to the Attune CytPix Flow Cytometer, the large-velocity brightfield camera captures and shops pictures of detected activities at a fee nearly 6,000 images for each 2nd, with regards to the flow fee and graphic dimension. For increased flexibility, Attune Cytometric Software helps you to alter the graphic seize frequency as necessary and select a particular gate for graphic capture, if essential.

Merge acoustic focusing engineering with substantial-parameter Investigation for versatile spectral unmixing and standard workflows.

Find new insights into your experimental facts by visualizing populations using combined fluorescence and morphology information. Our software program interface wizards enable downsampling, concatenation (merged data files) and dimensionality instruments that are built in the program for an intuitive, streamlined facts Evaluation working experience.

*Pricing is indicative only and is also subject to vary. Make sure you Call your Agilent representative with any concerns. The data established forth herein is not a proposal or a determination, both of those of which are issue to credit score acceptance, conditions and terms, and availability with Agilent. Agilent Fiscal Alternatives is on the market in selected nations.

Furthermore, it's illuminating to view what number of doublets slip by conventional singlet gates, and wonderful to confirm the position of unpredicted double-positive activities in my analysis."

Pair your Attune flow cytometer with the autosampler for greater workflow performance, and increase robotic integration for maximum throughput.

Constant picture top quality even at substantial throughput Acoustic focusing and a higher-speed digital camera Incorporate to picture these T cells continually at reduced or significant flow prices. Effortlessly alter focus and digicam options to meet experimental prerequisites.

Early signs of this contamination had in the beginning been dismissed as particles. Backgating to find out new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also enables you to use morphological characteristics to find out exciting subpopulations that may not be obvious from flow cytometry data alone.

Buyers can check out celebration illustrations or photos in true-time to confirm sample good quality, enhance protocols, and document the morphology of each mobile populace discovered for Investigation. Imaging seize also Added benefits from acoustic focusing, which helps you to placement the cells in order that a sharp, centered picture is received.

Audio waves help exact cell alignment, leading to Remarkable knowledge excellent and ~10X speedier sample flow costs.

Application note: Automatic picture Investigation decreases person-to-consumer variability in flow cytometry gating strategies

Leverage the strength of advanced technological know-how and innovation to speed up your discoveries. Rely on in the Attune Flow Cytometer family members to provide the dependability, velocity, and precision that established your get the job done aside.

No wash/no lyse leukocyte Examination with imaging. Cells ended up obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Skilled UX/UI permits users to teach AI types for expanded purposes. (A) The “Teach Model” aspect gives annotation instruments and serious-time product training accuracy.

At the heart of each and every Attune Flow Cytometer lies our groundbreaking acoustic concentrating know-how, which utilizes seem waves to meticulously align cells in the flow mobile. This modern technique surpasses classic hydrodynamic procedures, resulting in quicker processing speeds, Fantastic knowledge high-quality, and greater sample throughput.

The Attune website Xenith cytometer is usually a substantial-parameter spectral analyzer, giving both equally spectral unmixing and traditional flow abilities for extensive and multipurpose mobile Assessment.

Agilent Benefit Promise ensures you at the least ten years of instrument use through the day you buy. Otherwise, We'll credit you Together with the residual value of the program toward an upgraded design.

Here, we exhibit that by gating strategies using impression-based mostly quantitative parameters can distinguish interacting cells from coincident events to more accurately assess interacting cells.